• Membrane proteins by ESR

    Detergents vs. membrane vesicles vs. nanodisc

    Nanodisc-based approach to enhance DEER measurements

    We use spin-nanodisc to improve SNR of DEER signals for conventional membrane protein study. The new approach enables a significant improvement in the distance resolution obtainable from the DEER result by Tikhonov method.

     

    RSC Advances, 9 (2019) 9014-9021

     

    ESR reveals lipid-dependent conformational changes of membrane proteins

    Membrane proteins are incorporated in various environments, including detergent, membrane vesicles, and nanodisc. ESR distance measurements show that the protein behave differently with environments.