• Apoptosis

    Programmed cell death

    ESR & Fluorescence Techniques

    Membrane pores

    Although membrane pores are induced by the formation of BAX complexes at membrane, the pore size depends critically on lipid composition.

    Role of Cardiolipin in the BAX Activation

    Lipid-dependent BAX oligomers

    We identify the inactive versus active forms of membrane-associated BAX, only the latter of which can induce stable and large membrane pores that are sufficient in size to pass apoptogenic factors. We reveal that the presence of CL is crucial to promoting the association between BAX dimers, hence the active oligomers. Without the presence of CL, BAX dimers assemble into an inactive oligomer that lacks the ability to form stable pores in the membrane. This study suggests an important role of CL in determining the formation of active BAX oligomers.

    Apoptosis

    Mitochondria-mediated apoptosis

    One of the main topics in the Chiang Lab is about understanding the molecular details of how the BCL2 protein family regulates the mitochondria-dependent apoptosis. In particular, we are interested in the BAX-mediated cell death pathway.

    Protein & Cell Membrane

    Membrane protein & protein-membrane associated complexes

    Our recent study reveals a complete solution structure of apoptotic BAX protein oligomer. The results suggest an alternative pathway of apoptosis in which BAX oligomer formation occurs prior to membrane insertion.

    Conformational Heterogeneity

    Allosteric regulation of protein function

    The Chiang Lab reports the BAX-induced apoptosis can be initiated through a conformational selection mechanism.