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The CHIANG LAB

  • Home
  • Our lab
  • Members
  • Y.W. Chiang
  • Publications 
    • After 2016
    • 2010 − 2015
    • 2004 − 2009
  • Research 
    • Apoptosis
    • Water dynamics
    • Proteins
    • Nanochannel
    • Nanodisc/MP
    • Metal coordinations
  • Teaching
  • …  
    • Home
    • Our lab
    • Members
    • Y.W. Chiang
    • Publications 
      • After 2016
      • 2010 − 2015
      • 2004 − 2009
    • Research 
      • Apoptosis
      • Water dynamics
      • Proteins
      • Nanochannel
      • Nanodisc/MP
      • Metal coordinations
    • Teaching

    The CHIANG LAB

    • Home
    • Our lab
    • Members
    • Y.W. Chiang
    • Publications 
      • After 2016
      • 2010 − 2015
      • 2004 − 2009
    • Research 
      • Apoptosis
      • Water dynamics
      • Proteins
      • Nanochannel
      • Nanodisc/MP
      • Metal coordinations
    • Teaching
    • …  
      • Home
      • Our lab
      • Members
      • Y.W. Chiang
      • Publications 
        • After 2016
        • 2010 − 2015
        • 2004 − 2009
      • Research 
        • Apoptosis
        • Water dynamics
        • Proteins
        • Nanochannel
        • Nanodisc/MP
        • Metal coordinations
      • Teaching
      • Membrane proteins by ESR

        Detergents vs. membrane vesicles vs. nanodisc

        Structure and regulation of the BsYetJ calcium channel

        The endoplasmic reticulum (ER) serves as the major intracellular calcium store. The release of calcium from this store regulates a variety of cellular functions, hence playing a key role in regulating apoptosis. The transmembrane BAX inhibitor-1 motif-containing 6 (TMBIM6) is an evolutionarily conserved multifunctional ER membrane protein that mediates calcium homeostasis to protect against apoptosis and ER stress. We study a bacterial homolog of TMBIM6 and report several previously unidentified conformational substates involved in the calcium transport process. Our findings stimulate a model of calcium-release channel showing that membrane protein functioning requires the interconversion between distinct conformations in preexisting conformational equilibria.

         

        PNAS, 117 (2020) 30126-30134

         

        Nanodisc-based approach to enhance DEER measurements

        We use spin-nanodisc to improve SNR of DEER signals for conventional membrane protein study. The new approach enables a significant improvement in the distance resolution obtainable from the DEER result by Tikhonov method.

         

        RSC Advances, 9 (2019) 9014-9021

         

        ESR reveals lipid-dependent conformational changes of membrane proteins

        Membrane proteins are incorporated in various environments, including detergent, membrane vesicles, and nanodisc. ESR distance measurements show that the protein behave differently with environments.

      Chiang Lab@NTHU

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